Codisplay of Rhizopus oryzae and Candida rugosa Lipases for Biodiesel Production

In this study, we overcame the limitations of single-enzyme system catalysis by codisplaying Candida rugosa lipase 1 (CRL1) and Rhizopus oryzae (ROL) on cell surfaces whole-cell catalyst Pichia pastoris to produce biodiesel from tallow seed oil. We screened double antibiotic-resistant strains tributyrin plates, performed second electroporation based single-displayed ROL GS115/KpRS recombinants CRL1 GS115/ZCS obtained an ROL/CRL1 codisplay P. GS115 surfaces. The maximum activity GS115/pRCS recombinant was 470.59 U/g dried cells, which 3.9-fold 1.3-fold higher than that CRL1, respectively. When self-immobilized lipases were used as catalysts, rate methyl ester production harboring 1.4-fold with ROL. Therefore, synergetic codisplayed enzymes is alternative strategy.